Lin Wu, PhD
PhD Biochemistry, Hong Kong University of Science and Technology, 2012
My thesis work is about biochemical and structural characterization of Usher Syndrome I protein complexes. Mainly using biochemical and biophysical methods, I identified all interaction sites of five Usher syndrome I proteins, and got the molecular structures of all protein complexes by collaborating with protein structurists. Our research offers a reliable interaction network of Usher I proteins, which was debatable by then. And combined with published in vivo data, our biochemical characterization provides explanation about how the tip link is organized and why such a fragile machinery can sustain strong sound wave impacts.
Currently in the Littman Lab, I'm interested in studying key molecules that dictate Th17 cell development and its pathogenicity in autoimmune diseases.
1. Wu Lin, Pan Lifeng, Wei Zhiyi, and Zhang Mingjie. (2011). Structure of MyTH4-FERM Domains in Myosin VIIa Tail Bound to Cargo" Science 331(6018):757-60
2. Wu Lin, Pan Lifeng, Zhang Chuchu, and Zhang Mingjie. (2012). Large protein assemblies formed by multivalent interactions between cadherin23 and harmonin suggest a stable anchorage structure at the tip link of stereocilia” J Biol Chem. 28;287(40):33460-71
3. Pan Lifeng, Yan Jing, Wu Lin and Zhang Mingjie. (2009). Assembling Stable Hair-Cell Tip Link Complex via Multi-dentate Interactions between Harmonin and Cadherin23" PNAS. 106 5575-5580
4. Yan Jing, Pan Lifeng, Chen Xiuye, Wu Lin, Zhang Mingjie. (2010). The structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. PNAS. 107 4040-5